| Name | ML324 |
| Description | ML324(IC50=920 nM) is a specific inhibitor of jumonji histone demethylase (JMJD2). |
| Kinase Assay | The fluorescence resonance energy transfer (FRET)-based LANCE Ultra KinaSelect Ser/Thr kit is used to determine IC50 values for various CDK inhibitors. Briefly, a specific ULight MBP peptide substrate (50 nM final concentration) is phosphorylated by a CDK-cyclin pair in buffer (50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM dithiothreitol) containing ATP at the concentration of the Km values of the individual kinases for 1 h at room temperature. Subsequently, phosphorylation is detected by addition of specific Eu-labelled anti-phospho-antibodies (2 nM), which upon binding to the phosphopeptide give rise to a FRET signal. FRET signals are then recorded[1]. |
| In vitro | ML324 exhibits good Caco-2 cell permeability, and possesses excellent microsomal stability in the presence of both mouse and rat liver microsomes. ML324 demonstrates potent anti-viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection by inhibiting viral IE gene expression. [1] |
| In vivo | In a mouse ganglia explant model of latently infected mice, ML324 suppresses the formation of HSV plaques, and blocks HSV-1 reactivation. [1] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.72 mM), Sonication is recommended.
Ethanol : 3 mg/mL (8.59 mM), Heating is recommended.
DMSO : 40 mg/mL (114.47 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
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| Keywords | virus | simplex | proteins | periodontitis | periodontal | ML-324 | ML324 | ML 324 | Jumonji | JMJD2 | Inhibitor | inhibit | inflammation | immunity | human | HSV | HistoneDemethylase | Histone Demethylase | Herpes simplex virus | herpes | H3K9 | domain | disease | Cytomegalovirus | containing | CMV |
| Inhibitors Related | Phenytoin sodium | Levofloxacin hydrochloride | Ribavirin | L-Lysine | 2-Deoxy-D-glucose | Oxyresveratrol | Ofloxacin | Acyclovir sodium | Acyclovir | Thymidine | N4-Acetylcytidine | Danthron |
| Related Compound Libraries | Methylation Compound Library | Histone Modification Compound Library | Bioactive Compound Library | Chromatin Modification Compound Library | Anti-Viral Compound Library | Inhibitor Library | Stem Cell Differentiation Compound Library | NO PAINS Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Infection Compound Library | Anti-Cancer Compound Library |
United States
