| Name | Methyl-β-cyclodextrin |
| Description | Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound utilized as a solubilizer for hydrophobic substances in biological studies, and serves as a lipid raft inhibitor with cholesterol-lowering and potential antitumor activities. |
| Cell Research | PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1]. |
| Kinase Assay | PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined. |
| In vitro | METHODS: Five primary exudative lymphoma (PEL) cell lines, BCBL-1, BC-1, BC-3, TY-1, and GTO, were treated with Methyl-β-cyclodextrin (0-10 mM) for 24 h, and cell viability was measured by MTT assay.
RESULTS: Methyl-β-cyclodextrin dose-dependently inhibited the growth of PEL cells with IC50 between 3.33-4.23 mM. [1]
METHODS: Chicken hepatocellular carcinoma cells, LMH, were treated with Methyl-β-cyclodextrin (10 mM) for 1 h, then cholesterol (50 µg/mL) was added at different time points before, during, and after infection with FAdV-4, and the expression levels of the target proteins were detected by Western Blot.
RESULTS: When LMH cells were pretreated with increasing concentrations of Methyl-β-cyclodextrin prior to infection, penton protein levels decreased in a dose-dependent manner. [2] |
| In vivo | METHODS: To test the antitumor activity in vivo, Methyl-β-cyclodextrin (500 mg/kg) was intraperitoneally injected into NRJ mice bearing PEL tumor BCBL-1 once a day for twenty-one days.
RESULTS: Methyl-β-cyclodextrin significantly inhibited the growth and invasion of PEL cells without significant adverse effects. [1]
METHODS: To investigate the modulatory effects on collagen, Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) was intradermally injected into the SKH1 mouse twice a week for two months.
RESULTS: Methyl-β-cyclodextrin showed potent COL I up-regulatory activity resulting in increased skin thickness. [3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility Information | DMSO : 55 mg/mL, Sonication is recommended.
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| Keywords | β-Cyclodextrin methyl ethers | Methyl-β-cyclodextrin | Methylβcyclodextrin | Methyl-b-cyclodextrin | Methyl β cyclodextrin | Inhibitor | inhibit | b-Cyclodextrin methyl ethers |
United States
