| Name | Mepivacaine hydrochloride |
| Description | Mepivacaine hydrochloride (Mepivacaine HCl) is the hydrochloride salt form of mepivacaine, an amide derivative with local anesthetic properties. |
| Kinase Assay | Phosphatase Assay: Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described. |
| In vivo | The tests made in horse show greater diffusion of mepivacaine between adjacent synovial structures than assumed from previous anatomical, latex injection and contrast arthrographic studies. [1] Ultrasound provides a 37% reduction in the minimum effective anesthetic volume (MEAV50) of 1.5% mepivacaine required to block the sciatic nerve compared with neurostimulation in patients undergoing knee arthroscopy. [2] The use of 3% mepivacaine provides a shorter duration of anesthesia than the lidocaine formulations with epinephrine in the canines and premolars. [3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 3 mg/mL (10.6 mM)
Ethanol : 8 mg/mL (28.28 mM)
H2O : 52 mg/mL (183.9 mM)
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| Keywords | regional | inhibit | Mepivacaine hydrochloride | Sodium Channel | Mepivacaine | Na channels | local | infiltration | Inhibitor | Mepivacaine Hydrochloride | Na+ channels | neuronal | cell | depolarization | anesthetic | amide-type |
| Inhibitors Related | Phenytoin sodium | Lidocaine hydrochloride | L-Aspartic aicd sodium |
| Related Compound Libraries | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Drug Repurposing Compound Library | Neuroprotective Compound Library | Bioactive Compounds Library Max | Ion Channel Targeted Library | Anti-Cancer Drug Library |
United States
