| Name | Levoleucovorin Calcium |
| Description | Levoleucovorin Calcium (CL307782), a calcium salt of the folinic acid, is used in cancer chemotherapy as an adjuvant. |
| Kinase Assay | Chk1 Assay: For the Chk1 assay, the kinase domain is expressed in Sf9 insect cells, and a biotinylated cdc25c peptide containing the consensus Chk1/Chk2 phosphorylation site (*)(biotin-[AHX]SGSGS*GLYRSPSMP-ENLNRPR[CONH2]) is used as the substrate. A dilution series of CHIR-124 is mixed with a kinase reaction buffer containing a final concentration of 30 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM DTT, 4 mM EDTA, 25 mMβ-glycerophosphate, 5 mM MnCl2, 0.01% bovine serum albumin, 1.35 nM CHK1 kinase domain, 0.5 μM peptide substrate, and 1 AM unlabeled ATP, plus 5 nM 33Pγ-labeled ATP (specific activity = 2,000 Ci/mmol). Reactions and detection of the phosphate transfer are carried out by a radioactive method. Reactions are incubated at room temperature for 1 to 4 hours and the phosphorylated peptide captured on streptavidin-coated microtiter plates containing stop reaction buffer (25 mM EDTA [ethylenediaminetetraacetic acid], 50 mMHEPES, pH 7.5). Phosphorylated peptide is measured with the DELFIA TRF system using a Europium-labeled anti-phosphotyrosine antibody PT66. The concentration of CHIR-124 for IC50 is calculated using nonlinear regression with XL-Fit data analysis software. |
| Storage | keep away from direct sunlight,store at low temperature,store under nitrogen | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : Insoluble, The compound is unstable in solution, please use soon.
H2O : 10 mM, Sonication is recommended.
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| Keywords | Levoleucovorin Calcium | Levoleucovorin | Inhibitor | inhibit | CL-307782 | CL 307782 | antineoplastic | Antifolate | anticancer | 5-Fluorouracil |
| Inhibitors Related | Pemetrexed | Methotrexate | Folinic acid | Levomefolate calcium | Methotrexate disodium | Trimethoprim | Folinic acid calcium | Pyrimethamine | Diaveridine | Pemetrexed disodium | Folinic Acid Calcium Salt Pentahydrate | Trimethoprim lactate |
United States
