| Name | Hoechst 33258 trihydrochloride |
| Description | Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication. it also interferes with mitosis. |
| Cell Research | Solution preparation
a. Preparation of stock solution: Dissolve 10 mg in 5 mL DMSO; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting)
b. Preparation of Hoechst working solution
Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/mL; (Select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use)
c. Cell staining
1. Suspended cells (6-well plate)
a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL;
b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes;
c. Centrifuge at 400 g for 3-4 minutes at 4℃, and discard the supernatant;
d. Wash twice with PBS, 5 minutes each time;
e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer.
2. Adherent cells
a. Culture adherent cells on sterile coverslips;
b. Remove coverslips from culture medium and aspirate excess culture medium;
c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 3-10 minutes;
d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 55 mg/mL (103.02 mM), Sonication is recommended.
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| Keywords | Parasite | Inhibitor | inhibit | Hoechst 33258 trihydrochloride | Hoechst 33258 Trihydrochloride | HOE-33258 | HOE33258 | H-33258 Trihydrochloride | H33258 Trihydrochloride | H-33258 | H33258 | H 33258 Trihydrochloride | H 33258 | bisBenzimide H-33258 Trihydrochloride | bisBenzimide H33258 Trihydrochloride | bisBenzimide H-33258 | bisBenzimide H33258 | bisBenzimide H 33258 Trihydrochloride | bisBenzimide H 33258 |
| Inhibitors Related | Flubendazole | Kojic acid | Urethane | Hydroxychloroquine | Metronidazole | Nitazoxanide | Doxycycline | Chloroquine phosphate | Diethyltoluamide | Artemisinin | Benzyl benzoate | DL-Methionine |
| Related Compound Libraries | Anti-Parasitic Compound Library | Bioactive Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Anti-Infection Compound Library | Human Metabolite Library |
United States
