The chemical name of horn-sterol is 24 β- Methylcholesterol-5,7-en-3 β- Hydroxyl, also known as ergosterol, is a white or colorless bright lobular crystal or white crystalline powder. Ergosterol not only has unique physiological effects, but also is widely used in drug development. As an important component of fungal cell membrane, ergosterol has stable structure and strong specificity. It is more representative than glucosamine for measuring biomass. Therefore, the biomass of fungi can be measured by measuring the content of ergosterol. Chemical properties colorless acicular or flaky crystals. Soluble in ethanol, ether, benzene and chloroform, insoluble in water. Dissolve ergosterol in chloroform, ether or cyclohexane and other solvents, add it into a quartz glass flask, and prepare vitamin D by ultraviolet irradiation. Uses: It has the function of vitamin D2. It is used as a precursor for the production of vitamin D2, as well as an intermediate for the production of hormone drugs. It can be used as a biological precursor for the production of vitamin D2 for the use of cortisone. It exists in the cell membrane of fungi and some protozoa such as trypanosoma. Ergosterol can be used to study the function of antifungal drugs such as amphotericin B and its analogues, and the biosynthesis pathway of ergosterol in various fungi. The production method uses the preparation of yeast as the raw material to extract dry yeast powder through 60-80 mesh sieve, add three times of 82% - 84% ethanol to extract for 18-24 hours, keep it at 70 ℃ for 3 hours, keep stirring, cool it to below 30 ℃, filter, extract the filter residue twice, throw out ethanol, combine the extract, concentrate at 70 ℃ in vacuum, and the concentration time is not more than 24 hours to obtain the paste. The filter residue is used to extract RNA and yeast polysaccharide. Dry yeast or geotrichum candidum [ethanol] → [first 68-70 ℃, then below 30 ℃] extract [70 ℃ concentration] → preparation of crude ergosterol paste Add 5% - 10% water to the paste, add 3-5 times the amount of ether, vigorously stir for 2-3h, stand for 16-20h, discard the middle and lower layer of liquid, and place the upper layer of ether solution in an ice box at - 5 ℃ for 20-24h to separate ergosterol crystals. Paste [ether] → upper ether solution [- 5 ℃] → [20-24h] crude ergosterol. The production method is extracted from the yeast that synthesizes the product from glucose. security information
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