| Name | Doxycycline |
| Description | Doxycycline (Doxiciclina) belongs to the tetracycline class of antibiotics and is a broad-spectrum metalloproteinase (MMP) inhibitor with oral activity. Doxycycline has antibacterial activity and antitumor activity. |
| In vitro | METHODS: Twenty-two tumor cells were treated with Doxycycline for 48 h. Cell viability was measured by MTT.
RESULTS: Lung cancer cells were more sensitive to Doxycycline than most other cell lines. NCI-H446 and A549 cells were sensitive to Doxycycline with IC50s of 1.70 μM and 1.06 μM, respectively. [1]
METHODS: Human pancreatic cancer cells PANC-1 were treated with Doxycycline (10-40 μg/mL) for 8-24 h. The cell cycle was detected by Flow Cytometry.
RESULTS: Doxycycline caused the cells to arrest in the G1-S phase of the cell cycle. [2] |
| In vivo | METHODS: To detect anti-tumor activity in vivo, Doxycycline (50 mg/kg) was administered intraperitoneally once daily for fifteen days to BALB/c mice harboring human mammary carcinoma tumor 4T1.
RESULTS: Doxycycline delayed tumor growth and reduced the number of tumor-associated macrophages and blood vessels. [3]
METHODS: To assay antimicrobial activity in vivo, Doxycycline (40 mg/kg) was administered intraperitoneally to BALB/c mice exposed to B. pseudomallei 1026b twice daily for two weeks.
RESULTS: Doxycycline-treated mice survived 100% of the time, and no B. pseudomallei DNA was amplified from the lungs or spleens of the majority of the surviving mice. [4] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | 10% DMSO+90% Saline : 4 mg/mL (9 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
DMSO : 50 mg/mL (112.5 mM), Sonication is recommended.
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| Keywords | MMP | Matrix metalloproteinases | 293T | MCF 12A | antibacterial activity | G55 | Antibiotic | Inhibitor | U343 | LNT-229 | Doxycycline | anti-proliferative activity | Bacterial | inhibit | SVG |
| Inhibitors Related | Neomycin sulfate | Dimethyl sulfoxide |
| Related Compound Libraries | Anti-Parasitic Compound Library | Bioactive Compound Library | Drug Repurposing Compound Library | Antiparasitic Natural Product Library | Anti-Cancer Approved Drug Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
