Basic information Safety Related Supplier

ChemicalBook Product Catalog Chemical Reagents Organic reagents D-Luciferin potassium

1/1

D-Luciferin potassium NEW

$41.00 10mg

$74.00 50mg

$105.00 100mg

Min. Order
Purity99.93%
Cas No115144-35-9
Supply Ability10g
Update time2024-11-19

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

5YR

United States

show more

Chemical Properties

Product Name	D-Luciferin potassium
CAS No	115144-35-9
EC-No
Min. Order
Purity	99.93%
Supply Ability	10g
Release date	2024/11/19

Product Introduction

Bioactivity

Name	D-Luciferin potassium
Description	D-Luciferin potassium (D-Luciferin K Salt) is a popular bioluminescent substrate of luciferase in the presence of ATP.
Kinase Assay	Enolase activity assay is in the 2.0 mM MgSO4 and 400 mM KCl in the absence or presence of ENOblock or NaF, at 37°C by incubating pure enolase (3–9 U) in a buffer containing 50 mM imidazole-HCl (pH 6.8). The reaction is initiated by adding 1 μmol of 2-phospho-D-glycerate, and the OD is measured after 10 min of reaction time with a spectrophometer at 240 nm.
In vitro	METHODS: Preparation of D-Luciferin potassium for in vitro bioluminescence analysis: 1. Prepare 200× D-Luciferin reserve solution (30 mg/mL) with sterile water and mix gently until all dissolved. It can be used immediately after preparation or stored at -20℃. 2. Prepare 1× D-Luciferin (150 µg/mL) working solution in preheated tissue culture medium. 3. Aspirate the medium from the cultured cells. 4. Add 1× D-Luciferin solution to the cells before imaging. Incubate the cells at 37°C for a short period of time before imaging to increase signal.
In vivo	METHODS: Preparation of D-Luciferin potassium for in vivo bioluminescence analysis: 1. Prepare a fresh 15 mg/mL D-Luciferin stock solution in DPBS. Sterilize by filtering with 0.20 um filter. 2. Inject 10 µL/g of mouse body weight. 150 mg D-Luciferin/kg body weight per mouse. For example, for a mouse weighing 10 g, inject 100 µL to provide 1.5 mg D-Luciferin. 3. inject D-Luciferin intraperitoneally 10-15 min prior to imaging. fluorescein kinetic studies should be performed for eah animal model to determine peak signal times.
Storage	keep away from direct sunlight,store at low temperature,store under nitrogen \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 2.63 mg/mL (8.26 mM), Sonication is recommended. H2O : 50 mg/mL (157 mM), Sonication is recommended.
Keywords	inhibit \| DLuciferin potassium \| Firefly luciferin \| D-Luciferin \| D-Luciferin potassium \| D Luciferin potassium \| Beetle Luciferin \| Inhibitor

show more

Company Profile Introduction

You may like

Recommended supplier

MSDS|CAS|CAS DataBase|Pricacy|Terms|About Us|New Products| Contact us|Computer Version
Chemical Book