| 名称 | Chrysophanol |
| 描述 | Chrysophanol (Turkey Rhubarb) is an EGFR/mTOR pathway inhibitor, which can be found in rhubarb, and sorrel. |
| 细胞实验 | Chrysophanol (Chrysophanic Acid) is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The cells are seeded at 5×103 cells/mL in 96-well microplates and allowed to attach for 24 h. Chrysophanol (20, 50, 80 and 120 μM) is added to the medium at various concentrations up to 120 μM and for different durations. After treatment, cell cytotoxicity and/or proliferation is assessed by a Cell Counting Kit-8 (CCK-8). Briefly, highly water-soluble tetrazolium salt, WST-8, produces an orange colored water-soluble product, formazan. The amount of formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.CCK-8 (10 μL) is added to each well and incubated for 3 h at 37°C, then cell proliferation and cytotoxicity are assessed by measuring the absorbance at 450 nm using a microplate reader.Three replicated wells are used for each experimental condition[1]. |
| 体内活性 | Chrysophanol (CA) has been shown to counteract obesity in C57BL/6 mice induced by a high-fat diet (HFD). Through in vivo studies involving male C57BL/6J mice, it was discovered that Chrysophanol significantly mitigates weight gain associated with HFD consumption. Specifically, mice subsisting on an HFD without Chrysophanol treatment experienced a weight increase of 23.92 ± 1.74 g over 16 weeks. In contrast, mice treated with Chrysophanol exhibited a markedly lower weight gain of 16.72 ± 2 g in the same period, underscoring Chrysophanol's potential as an effective intervention against HFD-induced obesity. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 1 mg/mL, Sonication is recommended.
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| 关键字 | EGFR | Chrysophanol | Epidermal growth factor receptor | ErbB-1 | HER1 | inhibit | Inhibitor |
| 相关产品 | Osimertinib | Lidocaine Hydrochloride hydrate | Lapatinib | Afatinib Dimaleate | Erlotinib hydrochloride | Erlotinib | Neratinib | Chalcone | Osimertinib mesylate | Genistein | Khellin | Gefitinib |
| 相关库 | 抗癌天然产物库 | 植物来源化合物库 | 酪氨酸激酶分子库 | 天然产物库 | 苗药化合物库 | 药物功能重定位化合物库 | 抑制剂库 | FDA 上市激酶抑制剂库 | 抗癌药物库 | 药食同源库 |
United States
