Description of BIS-Tris from Watson Biotechnology Co., Ltd
Basic Information of BIS-Tris |
Product Name | BIS-Tris |
CAS NO. | 6976-37-0 |
Apperance | White powder |
Assay | 99% |
Pacakge | 1kg/Foil Bag,25kg/Drum/Box |
MOQ | 1kg |
Storage | Cool and dry place |
Shelf Life | 2 Years |
Brand | Watson |
Specifications(COA) of BIS-Tris CAS 6976-37-0 Buffering Agent
ITEMS | SPECIFICATIONS | RESULTS |
Appearance | White crystall powder | Conform |
Water content | ≦1.0 | 0.35 |
PH (1% aq.) | 9.5 – 11.0 | 9.55 |
Solubility (0.1M aq.) | Clear, cololess solution | Conform |
Ultraviolet absorbance(0.1M aq.) 280nm | ≦0.04 | 0.027 |
Ultraviolet absorbance(0.1M aq.)340nm | ≦0.02 | 0.014 |
Assay | 99.0 -101.0 | 100.23 |
Conclusion | QUALIFIED. |
BIS-Tris (N,N-Bis(2-hydroxyethyl)-2-amino-2-(hydroxymethyl)-1,3-propanediol) is a buffering agent commonly used in biochemical and molecular biology applications. Here are some of its primary applications:
1. **pH Buffering in Biological Systems**: BIS-Tris is widely used as a buffering agent because it can maintain a stable pH in solutions, particularly within the pH range of 5.8 to 7.2. This makes it suitable for experiments involving proteins, enzymes, and other biological molecules, which often require a specific pH to maintain their structure and function.
2. **Electrophoresis**: In polyacrylamide gel electrophoresis (PAGE), BIS-Tris is used as a buffer in both the gel and running buffer. It helps maintain the pH and conductance necessary for the separation of proteins or nucleic acids based on size and charge.
3. **Protein Purification and Crystallization**: BIS-Tris is used in protein purification protocols, especially in affinity chromatography and ion exchange chromatography. It helps in maintaining the stability and solubility of proteins. Additionally, it is used in protein crystallization trials due to its buffering properties and minimal interference with protein crystallization.
4. **Enzyme Reactions**: BIS-Tris is used in various enzyme assays where maintaining a stable pH is crucial for optimal enzyme activity. It helps in studying enzyme kinetics and other biochemical properties of enzymes.
5. **Cell Culture Media**: Although less common than other buffers, BIS-Tris can be used in cell culture media formulations to maintain a stable pH environment, which is critical for cell growth and metabolism.
6. **General Laboratory Use**: As a versatile buffer, BIS-Tris can be used in a wide range of laboratory experiments, including DNA and RNA work, protein studies, and other biochemical assays.
Overall, BIS-Tris is favored in many biochemical applications due to its good buffering capacity, minimal interference with biological processes, and compatibility with a wide range of analytical techniques.
FAQ From Watson Biotechnology Co., Ltd |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
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Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
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Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
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Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
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Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
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Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.
With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.
At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.
Watson Biotechnology Co., Ltd is a professional manufacturer which provide BIS-Tris with good quality and best price, welcome to contact us to get COA/TDS/MSDS of BIS-Tris.
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