BIS-TRIS NEW

$0.00 1kg

$0.00 25kg

Min. Order1kg
Purity99%-101%
Cas No6976-37-0
Supply Ability500 KG
Update time2025-12-30

Wuhan Fortuna Chemical Co., Ltd

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Chemical Properties

Product Name	BIS-TRIS
CAS No	6976-37-0
EC-No	230-237-7
Min. Order	1kg
Purity	99%-101%
Supply Ability	500 KG
Release date	2025/12/30

BIS-TRIS Cas 6976-37-0

Specification

Items	Specifications	Results
Appearance	White to almost white powder	White powder
Assay	99.0-101.0	99.89%
loss on drying	≤1%	0.24%
Solubility(0.1M aqueous)	Colorless, clear	Colorless, clear
PH(1% aqueous)	9.5-11.0	9.63
Ultraviolet absorbance/340nm(0.1M aqueous)	≤0.02	0.008
Ultraviolet absorbance/280nm(0.1M aqueous)	≤0.04	0.01
Conclusion	The product conforms to the above specifications.

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BIS-Tris (full name: 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol) is a zwitterionic organic compound widely used as a biological buffer in biochemical and molecular biology research. It is not a medication, unlike Empagliflozin, but an essential laboratory reagent.

Key Chemical Property: It is a "Good's Buffer" — part of a series of buffers developed in the 1960s to provide stable pH in biological systems without interfering with biochemical processes.

Primary Uses and Applications

BIS-Tris is valued for its stability and minimal interference, making it ideal for sensitive biochemical techniques.

1. Gel Electrophoresis (Most Common Use)

Purpose: To separate proteins or nucleic acids by size.
Why BIS-Tris? It forms a stable pH gradient during electrophoresis, especially in polyacrylamide gels.
Key Application: It is the preferred buffer for pre-cast NuPAGE® gels (commercial gel systems) used in western blotting and protein analysis. It provides superior resolution and sharper bands compared to older buffer systems like Tris-Glycine.

2. Protein Purification & Crystallization

Purpose: To maintain a stable pH environment for proteins during purification steps (e.g., chromatography) or while growing protein crystals for X-ray crystallography.
Why BIS-Tris? It has low UV absorbance, doesn't chelate metal ions strongly, and is less likely to interact with the protein of interest.

3. Cell Culture Media

Purpose: To help maintain a constant physiological pH (typically around 6.5-7.5) in cell culture media.
Why BIS-Tris? It is effective in the pH range relevant for many cell cultures and has low cell membrane permeability, meaning it stays outside the cell where it's needed.

4. General Laboratory Buffer

Used in various enzymatic assays, spectrophotometric studies, and as a component in reagent formulations where a consistent pH between 5.5 and 7.5 is required.

Key Advantages (Why Scientists Choose BIS-Tris)

Optimal pH Range: Effective pKa of ~6.5 at 25°C, making it perfect for many biological systems near physiological pH.
Minimal Interference: Low reactivity with metal ions, reducing the chance of inhibiting metal-dependent enzymes.
High Solubility: Highly soluble in water, allowing for the preparation of concentrated stock solutions.
Stability: Chemically stable, resistant to enzymatic degradation, and does not absorb visible or UV light significantly.
Isoelectric Focusing: Useful for creating immobilized pH gradients.

Comparison with Common Buffer "Tris"

It's crucial not to confuse BIS-Tris with the more common buffer Tris (tris(hydroxymethyl)aminomethane).

Feature	BIS-Tris	Tris
pKa at 25°C	~6.5	~8.1
Best pH Range	5.5 - 7.5	7.2 - 9.0
Common Use	Protein electrophoresis (mid-pH), cell culture	Nucleic acid electrophoresis, general-purpose high-pH buffering
Key Strength	Superior for protein structure preservation in gels	Standard, inexpensive buffer for DNA/RNA work

Safety and Handling

Form: Typically a white crystalline powder.
Safety: Generally considered low toxicity for laboratory use, but standard safety precautions (wearing gloves, eye protection, avoiding inhalation) should always be followed.
Preparation: Solutions are made by dissolving the powder in purified water (e.g., deionized water) and adjusting to the exact pH using HCl or NaOH.

In summary, BIS-Tris is a specialized, high-performance buffering agent that is a staple in modern biochemistry labs, particularly prized for its role in producing high-quality protein separation data.

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