| Name | Bay 11-7085 |
| Description | Bay 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM). |
| Cell Research | Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.(Only for Reference) |
| Kinase Assay | In Gel Kinase Assay: In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30?°C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control. |
| In vitro | In athymic nude mice harboring Caov-3 cells, BAY 11-7085 enhances the inhibitory effect of paclitaxel on intraperitoneal spread and ascites formation. Additionally, BAY 11-7085 suppresses the increase in NF-κB activity associated with meningitis, improves the clinical condition of infected rats, and significantly reduces CNS complications and inflammation related to meningitis. |
| In vivo | When used in conjunction with paclitaxel, BAY 11-7085 enhances the inhibition of NF-κB activity and reduces the survival rate of paclitaxel-treated cells. By inhibiting NF-κB, BAY 11-7085 suppresses the expression of the adhesion molecules E-selectin, ICAM-1, and VCAM-1 induced by TNFα. BAY 11-7085 (10 μM) exhibits no cytotoxicity towards HUVEC cells. Moreover, the combined use of BAY 11-7085 and LY294002 synergistically influences apoptosis in PEL cells. |
| Storage | keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 125 mg/mL (501.34 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (8.02 mM), Sonication is recommended.
Ethanol : 24.9 mg/mL (99.87 mM), Sonication is recommended.
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| Keywords | Nuclear factor-κB | Nuclear factor-kappaB | NF-κB | NFκB | NF-kB | NFkB | IκBα phosphorylation | IκB | Inhibitor | inhibit | IKK | IkB/IKK | IkB | Ferroptosis | BAY 11-7085 | Bay 11-7085 | Bay 117085 | Bay 11 7085 | Apoptosis |
| Inhibitors Related | Stavudine | Cysteamine hydrochloride | Sodium 4-phenylbutyrate | L-Ascorbic acid | L-Glutamic acid | Tributyrin | L-Cystine | L-Glutamine | N,N-Dimethylacetamide | L-Ascorbic acid sodium salt | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Highly Selective Inhibitor Library | Bioactive Compound Library | HIF-1 Signaling Pathway Compound Library | Hematonosis Compound Library | Inhibitor Library | NO PAINS Compound Library | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Anti-Liver Cancer Compound Library | Oxidation-Reduction Compound Library |
United States
