| 名称 | Bay 11-7085 |
| 描述 | Bay 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM). |
| 细胞实验 | Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.(Only for Reference) |
| 激酶实验 | In Gel Kinase Assay: In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30?°C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control. |
| 体外活性 | In athymic nude mice harboring Caov-3 cells, BAY 11-7085 enhances the inhibitory effect of paclitaxel on intraperitoneal spread and ascites formation. Additionally, BAY 11-7085 suppresses the increase in NF-κB activity associated with meningitis, improves the clinical condition of infected rats, and significantly reduces CNS complications and inflammation related to meningitis. |
| 体内活性 | When used in conjunction with paclitaxel, BAY 11-7085 enhances the inhibition of NF-κB activity and reduces the survival rate of paclitaxel-treated cells. By inhibiting NF-κB, BAY 11-7085 suppresses the expression of the adhesion molecules E-selectin, ICAM-1, and VCAM-1 induced by TNFα. BAY 11-7085 (10 μM) exhibits no cytotoxicity towards HUVEC cells. Moreover, the combined use of BAY 11-7085 and LY294002 synergistically influences apoptosis in PEL cells. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 24.9 mg/mL (100 mM)
Ethanol : 24.9 mg/mL (100 mM)
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| 关键字 | BAY 11-7085 | Apoptosis | NF-κB | inhibit | Nuclear factor-κB | Ferroptosis | Inhibitor | Bay 11 7085 | Bay 117085 | Nuclear factor-kappaB | Bay 11-7085 |
| 相关产品 | Stavudine | 5-Fluorouracil | Myricetin | Sodium 4-phenylbutyrate | L-Ascorbic acid | Dextran sulfate sodium salt (MW 4500-5500) | Metronidazole | Sorafenib | Tributyrin | Curcumin | L-Cystine | Diallyl disulfide |
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United States
