| 名称 | BAPTA-AM |
| 描述 | BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling. |
| 体外活性 | METHODS: Chondrocytes were treated with BAPTA-AM (10 μM) and FAC (100 μM) for 24 h. Intracellular ROS levels were measured using the Reactive Oxygen Species Assay kit.
RESULTS: FAC promoted ROS production and this effect was inhibited by the calcium chelator BAPTA-AM. [1]
METHODS: Rat fibroblast RAT2 and Xenopus cells were treated with BAPTA-AM (50 μM) for 1 h, and microtubule depolymerization was detected by Immunostaining.
RESULTS: BAPTA AM treatment for 30 min resulted in almost complete disassembly in most cells, and microtubules were uniformly depolymerized in cells within 60 min. [2] |
| 体内活性 | METHODS: To investigate the effect on ethanol-induced locomotor activity, BAPTA-AM (0-10 mg/kg, Cremophor EL 1.25% (v/v) in distilled water) was injected intraperitoneally into Swiss (RjOrl) mice, followed by ethanol (0-4 g/kg) 30 min later.
RESULTS: Pretreatment with BAPTA-AM blocked the locomotor stimulus produced by ethanol without altering basal locomotion. On the contrary, BAPTA-AM reversed the ethanol-induced hypnosis. [3]
METHODS: To investigate the effect on LPS-induced blood-brain barrier leakage, BAPTA-AM (12 mg/kg, 0.01% pluronic acid in sterile saline) was injected intravenously into FVB mice, followed 30 min later by intraperitoneal injection of LPS (25 mg/kg).
RESULTS: BAPTA-AM reduced LPS-induced blood-brain barrier leakage. [4] |
| 存储条件 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 50 mg/mL (65.39 mM), Sonication is recommended.
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| 关键字 | Potassium Channel | inhibit | Inhibitor | KcsA | BAPTA-AM | BAPTA AM | BAPTAAM |
| 相关产品 | Minoxidil sulfate | Glibenclamide | (±)-Naringenin | Tolbutamide | Tetraethylammonium bromide | Halothane | Butamben | Cloperastine hydrochloride | 2,2,2-Trichloroethanol | Indapamide |
| 相关库 | 铁死亡化合物库 | 疼痛相关化合物库 | 经典已知活性库 | 膜蛋白靶向化合物库 | NO PAINS 化合物库 | 已知活性化合物库 | 钾通道分子库 | 离子通道库 | 抗癌化合物库 | 人代谢物化合物库 |
United States
