Appearance: White or yellowish powder
Agarose, abbreviated AG, is the neutral, uncharged component of AGAR. The chemical structure of agarose is composed of β-D-galactopyranose (1-4) linked to 3, 6-dehydration alpha-L-galactopyranose units. Agarose, a polysaccharide AGAR that contains almost no sulfate, is dissolved in hot water and cooled to make a gel. The resulting small particles are used for gel filtration. It is suitable for the filtration of macromolecules that cannot be separated by sephadex. If the gel with a concentration below 5% is used, it can also separate cell particles, viruses, etc. Because of its low adsorbability, it is sometimes used instead of AGAR as a support for immunoelectrophoresis or gel deposition reactions.
Product function:
DNA, lipoprotein and immunoelectrophoresis; Substrate for biochemical studies such as immunodiffusion
AGAR pectin is a heterogeneous mixture of many smaller molecules. Agarose is generally heated to dissolve above 90 ° C in water, and a good semi-solid gel is formed when the temperature drops to 35-40 ° C, which is the main feature and basis for it to have a variety of uses. Agarose gel properties are usually an indicator of gel strength. The higher the strength, the better the gel performance.
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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