| Name | AFMK |
| Description | AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is a natural product and an apoptosis modulator with antioxidant and free radical scavenging activities. This compound enhances the antitumor effect of gemcitabine in PANC-1 cells and attenuates X-ray-induced oxidative damage to DNA, proteins, and lipids. |
| In vitro | Methods: Normal human epidermal keratinocytes were used as a model. AFMK was dissolved in ethanol and pre-incubated at concentrations of 10⁻³–10⁻⁹ M for 1 h, followed by 25 mJ/cm² UVB irradiation and 48 h culture. MTT, ATP, mitochondrial membrane potential, cytochrome c release, Caspase 3/7, and sub-G1 apoptosis assays were performed.
Results: AFMK significantly enhanced cell viability, maintained ATP and membrane potential, inhibited cytochrome c release, caspase activation, and apoptosis, exerting mitochondrial protective effects. [1]
Methods: Rat pancreatic acinar AR42J cells were used. Cells were incubated with 10⁻¹²–10⁻⁸ M AFMK combined with 10⁻⁸ M caerulein stimulation for 48 h. Western blot was used to detect GPx, TNF-α, Bcl-2, Bax, and Caspase-3 protein expression.
Results: AFMK dose-dependently increased GPx, Bax, and Caspase-3 levels, decreased TNF-α and Bcl-2 levels, and exerted protective effects by regulating antioxidant and apoptosis pathways.[2] |
| In vivo | Methods: In male Wistar rats, an acute pancreatitis model was induced by subcutaneous infusion of caerulein. AFMK (5, 10, 20 mg/kg) was administered by single intraperitoneal injection 30 min before modeling, with solvent being saline plus absolute ethanol; the control group received an equal volume of solvent.
Results: AFMK dose-dependently alleviated pancreatic tissue edema, reduced serum amylase and TNF-α levels, validating its in vivo anti-inflammatory and antioxidant activities.[3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (189.19 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (7.57 mM), Sonication is recommended.
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| Keywords | Human Endogenous Metabolite | Freeradicalscavengers | Free radical scavengers | EndogenousMetabolite | Endogenous Metabolite | Apoptosis | Antioxidant | AFMK |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Malic acid | Formamide | Glycerol | Thymidine | Corn starch | Gluconate Calcium | DL-Lysine |
| Related Compound Libraries | Apoptosis Compound Library | Bioactive Compound Library | Antioxidant Compound Library | Anti-Aging Compound Library | Human Endogenous Metabolite Compound Library Plus | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Human Metabolite Library | Anti-Cancer Active Compound Library |
United States
