| Name | AEBSF hydrochloride |
| Description | AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin. |
| Cell Research | The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm. |
| In vitro | In a mouse model of cockroach allergen-induced asthma, AEBSF was able to mitigate respiratory reactions and potential inflammation. Intraperitoneal injection of 76.8 mg/kg AEBSF daily in mice lethally inoculated with Toxoplasma gondii extended their survival period. |
| In vivo | AEBSF is a serine protease inhibitor that suppresses the lysis of leukemia cells by macrophages without inhibiting the secretion of TNF-α and IL-1β by these cells. In five different human cell lines, AEBSF inhibits the formation of amyloid-beta (Aβ) by blocking the secretion of both neural and non-neural β-secretase products. Additionally, AEBSF disrupts embryonic growth within the endometrium and modifies protein secretion mechanisms to prevent the adhesion of HeLa cells in human umbilical vein endothelial cells. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (20.86 mM), Sonication is recommended.
H2O : 24 mg/mL (100.13 mM), Sonication is recommended.
DMSO : 247 mg/mL (1030.5 mM), Sonication is recommended.
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| Keywords | Thrombin | Threonine proteases | SerineProtease | Serine proteases | Serine Protease | Serine endopeptidases | Ser/Thr Protease | Inhibitor | inhibit | InfluenzaVirus | Influenza Virus | AEBSF hydrochloride | AEBSF Hydrochloride | AEBSF |
| Inhibitors Related | Heparin sodium salt | α-Vitamin E | Molnupiravir | Umifenovir hydrochloride | Nitazoxanide | Camphor | Curcumin | Naringenin | Salcomine | p-Hydroxycinnamic acid | Crystal Violet | β-Cyclodextrin |
| Related Compound Libraries | Highly Selective Inhibitor Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Anti-Viral Compound Library | Inhibitor Library | NO PAINS Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Fluorochemical Library | Covalent Inhibitor Library | Anti-Infection Compound Library | Anti-Metabolism Disease Compound Library |
United States
