1. Materials information
Names
| Name |
acridine orange
|
| Synonym |
More Synonyms
|
Acridine Orange hydrochloride Biological Activity
| Description |
Acridine orange is a cell-permeable fluorescent dye that binds to
nucleic acids, resulting in an altered spectral emission. |
| Related Catalog |
Signaling Pathways >>
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Research Areas >>
Others
|
| In Vitro |
Acridine orange has been employed extensively as a cytochemical
stain and has shown to stain differentially DNA and RNA, and
double-restranded nucleic acids in situ. Acridine orange can either
intercalate into double helical nucleic acids (green fluorescence at 530
nm), or bind electrostatically to phosphate groups of single-stranded
molecules (red fluorescence at 640 nm). This unique characteristic makes
acridine orange useful for cell-cycle studies[1]. Acridine orange
staining of unfixed cells may be used as a simple, fast means of
obtaining information on cell ploidy levels and cell cycle status from
DNA measurements (green fluorescence), and cell transcriptional activity
from RNA staining (red fluorescence), in human and murine cells lines,
peripheral blood and bone marrow specimens from patients with leukemia
and mitogenically (phytohemagglutinin) or antigenically (mixed
lymphocyte culture) stimulated human peripheral blood cultures[2].
|
| Cell Assay |
Two-step, pH 3.0: Aliquots (0.2 mL, containing approximately
2-5x105 cells) are withdrawn from cultures and are added to 0.5 mL of a
solution containing: 0.1% (v/v) Triton X-100, 0.2 M sucrose, 10-4 M EDTA
and 2x10-2 M citrate-phosphate buffer, at pH 3.0. Triton X-100 is
included in the various procedures at the indicated pH to increase cell
permeability yet maintain cellular integrity. The chelating agent EDTA
is used to facilitate RNA denaturation. The cells are stained one minute
later by addition of 1 mL of a solution containing 0.002% (20 μg/mL)
AO, 0.1 M NaCl and 10-2 M citrate-phosphate buffer, pH 3.8. Cations are
included in the staining mixture to ensure staining specificity. The
final AO concentration is approximately 4x10-5 M[2].
|
| References |
[1]. McMaster
GK, et al. Analysis of single- and double-stranded nucleic acids on
polyacrylamide and agarosegels by using glyoxal and acridine orange.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos
F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic
acids in unfixed cells using acridine orange in a flow cytofluorometric
system. J Histochem Cytochem. 1977 Jan;25(1):46-56.
|
Chemical & Physical Properties
| Density |
1.001 g/mL at 20ºC |
| Boiling Point |
468.6ºC at 760 mmHg |
| Melting Point |
284-287ºC(lit.) |
| Molecular Formula |
C17H20ClN3 |
| Molecular Weight |
301.81400 |
| Flash Point |
237.2ºC |
| Exact Mass |
301.13500 |
| PSA |
19.37000 |
| LogP |
4.32200 |
| Index of Refraction |
n20/D 1.338 |
| Water Solubility |
Soluble in water, ethanol, dimethyl sulfoxide |
MSDS
|
Acridine Orange hydrochloride MSDS(Chinese)
|
Toxicological Information
CHEMICAL IDENTIFICATIONCAS REGISTRY NUMBER : 65-61-2
MOLECULAR FORMULA : C17-H19-N3.Cl-H
HEALTH HAZARD DATAACUTE TOXICITY DATATYPE OF TEST : Micronucleus test
MUTATION DATAREFERENCE : BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New
York, NY 10158) V.1- 1963- Volume(issue)/page/year: 11,2537,1972 ***
NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL
EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey
(1983) NOES Hazard Code - X3803 No. of Facilities: 29 (estimated) No. of
Industries: 1 No. of Occupations: 3 No. of Employees: 625 (estimated)
No. of Female Employees: 403 (estimated) NOES - National Occupational
Exposure Survey (1983) NOES Hazard Code - X8883 No. of Facilities: 10
(estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees:
114 (estimated) No. of Female Employees: 31 (estimated)
|
Safety Information
| Personal Protective Equipment |
Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US) |
| Safety Phrases |
S22-S24/25
|
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
3 |
| RTECS |
AR7601000 |
| HS Code |
32041300 |
2. Packaging of materials
For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.
For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product.
Or smaller package 1kg/bottle, 10kgs/bottle as request.
3. Shipping & Delivery
Provide door to door service
Suitable for goods under 50kg
Delivery: 3-7 days
Cost: low cost
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Suitable for goods over 50kg
Delivery: 3-14 days
Cost: high cost
Provide seaport to seaport service
Suitable for goods over 100kg
Delivery: 2-45 days
Cost: low cost
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