Normal horse serum may be used as a blocking agent and controls in immunoassays. For immunohistochemistry and immunocytochemistry, 10% horse serum was used as blocking agent in various Studies.
Horse serum was used in immunostaining for blocking non-specific antibodies binding.
Horse serum displays homogeneity between fetal bovine serum and fetal calf serum .It has low levels of polyamine oxidase.
The horse serum is an alternative to bovine serum for growth factors and hormones for cell culture studies. Horse serum stimulates fibroblast differentiation and extracellular matrix (ECM) and cytokines synthesis.