溴百里酚蓝
溴百里酚蓝 性质
熔点 | 200-202 °C (lit.) |
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密度 | 1.4668 (estimate) |
闪点 | 38 °C |
储存条件 | Store at +5°C to +30°C. |
溶解度 | 微溶于水、苯;溶于乙醇、乙醚;不溶于石油醚 |
酸度系数(pKa) | 7.0, 7.1(at 25℃) |
沸点 | 614.3±55.0 °C(Predicted) |
形态 | 固体 |
颜色 | 粉棕色至紫色 |
气味 (Odor) | 特异气味 |
PH值 | pH : 6.0~7.6 |
酸碱指示剂变色ph值范围 | 6(yellow)-7.6(blue) |
水溶解性 | slightly soluble |
最大波长(λmax) | 420nm, 435nm, 620nm |
Merck | 14,1445 |
BRN | 373934 |
稳定性 | 稳定的。与强氧化剂不相容。 |
主要应用 | Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system, paints, toys, cleaning products, detergents, textiles, food freshness sensor, identifying fresh and stale rice,wheat, detecting microorganisms, bacterial growth, psychoactive drug, dental materials |
LogP | 8.600 (est) |
CAS 数据库 | 76-59-5(CAS DataBase Reference) |
EPA化学物质信息 | Bromothymol blue (76-59-5) |
溴百里酚蓝 用途与合成方法
溴百里酚蓝呈无色或浅玫瑰结晶性粉末状,易溶于醇、稀碱溶液和氨水中,微溶于水,不溶于石油醚。其遇酸性时为黄色,碱性时为蓝色。
溴麝香草酚蓝是一种酸碱指示剂,变色范围pH6.0(黄)~7.6(蓝)。普通水是中性,pH也就是7左右,差不多呈浅绿。
溴百里酚蓝是弱酸和弱碱的pH指示剂,在分析化学中用作酸碱指示剂和色谱分析试剂;也可用作染料和代谢产物。
pH变色范围为6.0 (黄色)~7.6(蓝色)。• pH指示剂 — 在近中性时具有精确的颜色转换,酸性时为黄色,碱性时为蓝色。
• 染色剂 — 在显微镜下追踪淋巴液的运动,确定细胞壁或细胞核
• 培养基组分 — 在琼脂凝胶培养基中,用于蜡样芽孢杆菌计数;作为细菌生长组分和检测底物,例如胱氨酸/乳糖/电解质缺乏
• BTB纸片法 — 应急测定全血胆碱酯酶活力,用于有机磷中毒的判断。
方法1:在搅拌下,在百里酚蓝的冰醋酸悬浮液中通入溴,反应在50℃下进行,可制得溴百里酚蓝。
方法2:在搅拌下将过量的溴、冰醋酸溶液慢慢加到百里酚蓝的水醋酸溶液中,于40~50℃下反应4h反应结束后,冷却至20℃以下,过滤,干燥即得无色溴百里酚蓝。
Bromothymol Blue 是一种 pH 指示剂,是测定弱酸弱碱时使用的一种指示剂,其酸性时为黄色,碱性时为蓝色。A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies.
安全信息
危险品标志 | Xn |
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危险类别码 | 10-41-36/37/38-20/21/22 |
安全说明 | 24/25-39-26-36 |
危险品运输编号 | 1170 |
WGK Germany | 3 |
RTECS号 | SJ450000 |
TSCA | Yes |
危险等级 | 3 |
包装类别 | III |
海关编码 | 29349990 |
溴百里酚蓝 价格(试剂级)
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024-11-11 | XW07659501 | 溴百里香酚蓝(BTB) | 76-59-5 | 5G | 54 |
2024-11-08 | A17746 | 溴百里酚蓝 | 76-59-5 | 10g | 431 |