化合物 T11896
![化合物 T11896 结构式](https://img.chemicalbook.com/CAS/20210111/GIF/2449093-46-1.gif)
化合物 T11896 性质
沸点 | 718.4±60.0 °C(Predicted) |
---|---|
密度 | 1.317±0.06 g/cm3(Predicted) |
储存条件 | Store at -20°C |
溶解度 | DMSO:135 mg/mL(259.56 mM;需要超声波) |
酸度系数(pKa) | 4.28±0.10(Predicted) |
形态 | 固体 |
颜色 | 浅黄至黄色 |
化合物 T11896 用途与合成方法
IC50: 24.5 µM (ATG4B); Kd: 16 μM (ATG4B)
LV-320 (0-120 µM; SKBR3, MCF7, JIMT1, and MDA-MB-231 cells) treatment results in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines.
LV-320 (120 µM; 48 hours; MDA-MB-231 cells) treatment results in an increase in LC3B-II, indicating that LV-320 blocks autophagic flux.
Western Blot Analysis
Cell Line: | SKBR3, MCF7, JIMT1, and MDA-MB-231 cells |
Concentration: | 0 µM, 25 µM, 50 µM, 75 µM, 100 µM, or 120 µM |
Incubation Time: | |
Result: | Resulted in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines. |
Cell Autophagy Assay
Cell Line: | MDA-MB-231 cells |
Concentration: | 120 µM |
Incubation Time: | 48 hours |
Result: | Blocked autophagic flux. |
LV-320 (100-200 mg/kg; oral gavage; three times over two days; GFP-LC3 mice) treatment results in a terminal blood level of 169 µM and a liver level of 104 µM. The expression of GFP-LC3 puncta is significantly greater accumulation in LV-320 treated animals compared to controls. LC3B-II protein is also increased in LV-320-treated animals. The treatment do not cause significant toxicity in mice at either dose.
Animal Model: | GFP-LC3 mice (females, 9-14 weeks) |
Dosage: | 100 mg/kg or 200 mg/kg |
Administration: | Oral gavage; three times over two days (Pharmacokinetic study) |
Result: | Terminal blood levels were 169 µM and liver levels were 104 µM. LC3B-II protein level was also increased. |