Tris Buffered Saline, with Tween? 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.
Biochem/physiol Actions
Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.
