Z-YVAD-AFC (trifluoroacetate salt)

Z-YVAD-AFC is a fluorogenic substrate for caspase-1.1,2 Upon enzymatic cleavage by caspase-1, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-1 activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.WARNING This product is not for human or veterinary use.

[1] NANCY A. THORNBERRY. A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes[J]. Nature, 1992, 356 6372: 768-774. DOI: 10.1038/356768a0
[2] M MARCELLI. Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP.[J]. Cancer research, 1998, 58 1: 76-83.