Ac-LEHD-AFC (trifluoroacetate salt)

Ac-LEHD-AFC is a fluorogenic substrate that can be cleaved by caspase-4, -5, and -9.1 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.WARNING This product is not for human or veterinary use.

[1] N A THORNBERRY. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.[J]. The Journal of Biological Chemistry, 1997, 272 29: 17907-17911. DOI: 10.1074/jbc.272.29.17907