O4I1 is an inducer of Oct3/4 expression and translation in diverse human cell lines (increases Oct3/4 mRNA in HEK293 cells by 2.5- and 4-fold at 10 and 20 μM, respectively). It also induces Oct3/4 expression and translation in terminally differentiated human fibroblasts. Through this action, O4I1 promotes the expression of pluripotency-associated genes in human neonatal foreskin fibroblasts.
O4I1 is a potent Oct3/4 inducer. It promotes expression and stabilization of Oct3/4, and also enhanced its transcriptional activity in human somatic cells, making it useful in regenerative medicine.
