PFPI may be used to incubate the lyophilized samples, for derivatization during GC/MS analysis to measure total d-chiro-Inositol content absorbed by rodents.
Derivatization before a GC analysis helps in yielding distinctive products, which can be achieved using a derivatizing reagent that reacts with amino and hydroxyl functionalities quantitatively. 1-(Pentafluoropropionyl)imidazole (PFPI) is a derivatizing reagent which was found to give high yield of products than pentafluoropropionic anhydride during preparation of methyl ester/pentafluoropropionyl derivatives.
reagent type: derivatization reagent
reaction type: Esterifications