Nuclear Extraction Kit

Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman’s Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman’s Transcription Factor Assay kits. The proteins isolated using this kit can also be used in electrophoretic mobility shift assays (EMSA) and western blotting applications.

[1] JUN FANG. Functional characterization of a multi-cancer risk locus on chr5p15.33 reveals regulation of TERT by ZNF148.[J]. ACS Combinatorial Science, 2017: 15034. DOI: 10.1038/ncomms15034
[2] SUSHAMA KAMARAJUGADDA. Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation.[J]. Oncotarget, 2016: 47674-47686. DOI: 10.18632/oncotarget.10274
[3] HAINING ZHANG. Liganded peroxisome proliferator-activated receptors (PPARs) preserve nuclear histone deacetylase 5 levels in endothelin-treated Sprague-Dawley rat cardiac myocytes.[J]. ACS Applied Bio Materials, 2014: e115258. DOI: 10.1371/journal.pone.0115258
[4] YOON HEE CHO. Therapeutic effects of water soluble danshen extracts on atherosclerosis.[J]. Evidence-based Complementary and Alternative Medicine, 2013, 2013: 623639. DOI: 10.1155/2013/623639
[5] HUI LI. Yohimbine enhances protection of berberine against LPS-induced mouse lethality through multiple mechanisms.[J]. PLoS ONE, 2012: e52863. DOI: 10.1371/journal.pone.0052863