Indo-1 AM is a cell-permeant acetoxymethyl ester of indo-1, a ratiometric fluorescent calcium indicator. Indo-1 AM is hydrolyzed by intracellular esterases to release Indo-1 in cells. Indo-1 is commonly used in flow cytometry. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.
Cell-permeant derivative of Indo 1. Indo 1-AM is fluorescent probe for Ca2+. After crossing the membrane, the product is rapidly hydrolyzed by cytoplasmic esterases to the membrane impermeant INDO 1. Indo 1-AM is possible using for ratiometric fluorescenc
INDO 1/AM is a cell permeable derivative of INDO 1.
Cell permeable fluorescent probe for Ca2+; lower affinity than FURA2; gives a large shift in fluorescent emission from 482nm to 398nm upon Ca2+ binding.
Excitation / Emission Maximum
349-364 nm/475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound)
