4-Methylumbelliferyl oleate

4-Methylumbelliferyl (4-MU) oleate is a fluorogenic substrate for acid and alkaline lipases. It is cleaved by lipases, liberating 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

Colourless Oil

4-Methylumbelliferyl oleate has been used:

• as a substrate to assay the activity of non-specific esterases
• to determine lipase activity
• as a substrate to evaluate pancreatic lipase inhibitory activity

4-Methylumbelliferyl oleate can act as Fatty acyl esters for use as fluorogenic substrates to continuously follow the hydrolytic activity of carboxylesterases, such as acid and alkaline lipases.

Fluorogenic substrate for lipases.

[1] M L ARMSTRONG. Lipid metabolism in xanthomatous skin of hypercholesterolemic rabbits.[J]. American Journal of Pathology, 1986, 125 2: 339-348.
[2] BERNARD J MONCLA K M P. Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes.[J]. ACS Applied Energy Materials, 2009: 78. DOI: 10.1186/1471-2180-9-78
[3] AKIHIRO OGAWA. Lactobacillus gasseri SBT2055 suppresses fatty acid release through enlargement of fat emulsion size in vitro and promotes fecal fat excretion in healthy Japanese subjects.[J]. Lipids in Health and Disease, 2015, 14: 20. DOI: 10.1186/s12944-015-0019-0

330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4)/445-454 nm