AF 488 C6 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 488 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.
AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for sensitive proteins and antibodies labeling. The dye is useful for numerous demanding applications, including microscopy.
AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products otherwise. We offer an isomerically pure 5-AF 488.
