Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used to detect the immunoreactive mass of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). It has also been used in western blotting.
Glucose 6-phosphate dehydrogenase (G6PD) is an enzyme encoded by X-linked gene, also called as housekeeping gene in mammals. This gene is localized on the distal long arm of the X chromosome q28, with 13 exons spanning 20kb. This enzyme is a dimer and each monomer contains 515 amino acids with a molecular weight of 59,256Da. The active enzyme occurs in a dimeric form with bound NADP. The binding site for NADP is located at amino acid 205.
G6PD (glucose-6-phosphate dehydrogenase) enzyme catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, reducing NADP to NADPH. It therefore, catalyzes the first step of hexose monophosphate pathway (HMP). As it oxidizes glucose-6-phosphate, it confers protection against oxidative damage in erythrocytes. Its deficiency is highly heterogeneous, with around 190 variants being reported. The deficiency of G6PD was discovered while studying hemolytic anemia. G6PD also results in hereditary nonspherocytic hemolytic anemia.
