Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase activity. HEPC is a truncated analog of phosphatidylcholine with the entire
sn-
1 substituent deleted, and with a hexadecylthioester in the
sn-
2 position.
1 HEPC is a substrate for Type II phospholipase A
2 (PLA
2) enzymes, such as porcine pancreatic, bee venom, and snake venom PLA
2. The intrinsic biological activity of HEPC has not been actively investigated.
