Histone H4R3Me2s (1-21)-GGK-biotin (trifluoroacetate salt)

Histone H4R3Me2s (1-21)-GGK-biotin is a peptide fragment of histone H4 that corresponds to amino acid residues 2-22 of the human H4 sequence. It is symmetrically dimethylated at arginine 3 and biotinylated via a C-terminal GGK linker. Symmetric dimethylation of histone 4 at arginine 3 is associated with transcriptional repression.1 It can recruit the DNA methyltransferase DNMT3a, leading to DNA methylation and further transcriptional repression. Histone H4R3Me2s (1-21)-GGK-biotin has been used as a substrate for the qualitative measurement of jumonji domain containing 1B (JMJD1B) arginine demethylase activity and in dot blot assays to verify antibody specificity.2WARNING This product is not for human or veterinary use.

[1] ROMÉO S. BLANC S R. Arginine Methylation: The Coming of Age.[J]. Molecular Cell, 2017, 28 1: 8-24. DOI: 10.1016/j.molcel.2016.11.003
[2] SIHUI LI. JMJD1B Demethylates H4R3me2s and H3K9me2 to Facilitate Gene Expression for Development of Hematopoietic Stem and Progenitor Cells.[J]. Cell reports, 2018, 23 2: 389-403. DOI: 10.1016/j.celrep.2018.03.051