HEXANUCLEOTIDE MIX

Hexanucleotide Mix is a mixture of hexanucleotides of all possible sequences for random-primed DNA labeling.
Labeled DNA probes with high specific activity are used in a variety of hybridization techniques:

• Screening of gene libraries
• Southern and northern blots
• In situ hybridizations
• RT-PCR
• Generation of cDNA libraries
• Synthesis of first-strand cDNA
• in the determination of vector titer
• Second strand synthesis

Convenient oligonucleotide mixture for rapid random-primed labeling of DNA with radioactive, digoxigenin- or biotin-labeled nucleotides. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.