Uses
Hexanucleotide Mix is a mixture of hexanucleotides of all possible sequences for random-primed DNA labeling.
Labeled DNA probes with high specific activity are used in a variety of hybridization techniques:
- Screening of gene libraries
- Southern and northern blots
- In situ hybridizations
- RT-PCR
- Generation of cDNA libraries
- Synthesis of first-strand cDNA
- in the determination of vector titer
- Second strand synthesis
General Description
Convenient oligonucleotide mixture for rapid random-primed labeling of DNA with radioactive, digoxigenin- or biotin-labeled nucleotides. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
