NBD Sphingosine

NBD sphingosine is a biologically active derivative of sphingosine that is tagged with a fluorescent nitrobenzoxadiazole (NBD) group. It has been used to study the cellular localization and metabolism of sphingosine in HeLa cells. NBD sphingosine inhibits proliferation of HCT116, PC3, HEK293T, and HFE-145 cells (IC₅₀s = 25.6, 40.5, 10.4, and 12.5 μM, respectively).

NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases[1][2].

[1] Bandhuvula P, Fyrst H, Saba JD. A rapid fluorescence assay for sphingosine-1-phosphate lyase enzyme activity. J Lipid Res. 2007 Dec;48(12):2769-78. DOI:10.1194/jlr.D700010-JLR200
[2] Lima S, Milstien S, Spiegel S. A real-time high-throughput fluorescence assay for sphingosine kinases. J Lipid Res. 2014 Jul;55(7):1525-30. DOI:10.1194/jlr.D048132