Thrombin Receptor Agonist Peptide (trifluoroacetate salt)

Thrombin receptor agonist peptide (TRAP-14) is a 14-amino acid peptide agonist of the α-thrombin receptor.1 It induces aggregation of washed platelets as well as platelets in citrated and hirudin plasma.2 TRAP-14 (100 μM) increases the cytosolic calcium concentration in isolated guinea pig pulmonary smooth muscle cells 5-fold over baseline.1 It increases pulmonary arterial pressure in isolated guinea pig lung when used at a concentration of 1 μM, which is comparable to the effect induced by 10 nM α-thrombin. TRAP-14 also induces contraction of isolated rat aortic rings and increases endothelin-1 (ET-1) levels in a dose-dependent manner, an effect that is reversed by the ETA antagonist BQ-123 and the nitric oxide synthase (NOS) inhibitor L-NNA (Item No. 80220).3WARNING This product is not for human or veterinary use.

[1] H LUM. Thrombin receptor activation peptide induces pulmonary vasoconstriction.[J]. American Journal of Physiology, 1994, 266 2 Pt 1: C448-54. DOI: 10.1152/ajpcell.1994.266.2.c448
[2] E GLUSA. Thrombin receptor activating peptide-induced cellular effects: comparative studies on human platelet activation and endothelium-dependent relaxation of porcine pulmonary arteries.[J]. Agents and actions. Supplements, 1995, 45: 303-307. DOI: 10.1007/978-3-0348-7346-8_41
[3] H I MAGAZINE  H R Y  O Butt. Endothelin and nitric oxide release modulate aortic contraction to selected thrombin receptor agonists.[J]. American Journal of Physiology, 1996, 270 6 Pt 1: C1815-8. DOI: 10.1152/ajpcell.1996.270.6.c1815