8,12-
iso-iPF
2α-VI is an isoprostane produced by non-enzymatic, free radical-induced peroxidative damage to membrane lipids. It is the most abundant isoprostane product that is formed during lipid peroxidation and is used as a biomarker for oxidative stress.
1 8,12-
iso-iPF
2α-VI is detected after CCl
4-induced oxidative damage to hepatic tissue and has been found elevated in the urine, blood, and cerebrospinal fluid of patients with Alzheimer’s disease.
2,3,4
8,12-iso-iPF2α-VI is a F2-isoprostanes. 8,12-iso-iPF2α-VI is a sensitive and specific marker of in vivo lipid peroxidation. 8,12-iso-iPF2α-VI can be used as a biomarker of oxidative damage in alzheimer's disease[1].
1. Li, H., Lawson, J.A., Reilly, M., et al. Quantitative high performance liquid chromatography/tandem mass spectrometric analysis of the four classes of F2-isoprostanes in human urine Proc. Natl. Acad. Sci. USA 96(23),13381-13386(1999).
2. Sicilia, T., Mally, A., Schauer, U., et al. LC-MS/MS methods for the detection of isoprostanes (iPF2α-III and 8,12-iso-iPF2α-VI) as biomarkers of CCI4-induced oxidative damage to hepatic tissue J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 861(1),48-55(2008).
3. Praticò, D., Clark, C.M., Liun, F., et al. Increase of brain oxidative stress in mild cognitive impairment. A possible predictor of Alzheimer disease Arch. Neurol. 59,972-976(2002).
4. Shaw, L.M., Korecka, M., Clark, C.M., et al. Biomarkers of neurodegeneration for diagnosis and monitoring therapeutics Nat. Rev. Drug Discov. 6,295-303(2007).
