Cy5 azide is a reactive dye containing an azide group which can participate in Click-Chemistry reactions. Cyanine5 is suitable for many different methods of fluorescence detection and can be easily detected in small quantities (nM) by naked human eye.
Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 Lmol1cm1
Quantum yield (Φ): 0.2
Cyanine5 azide chloride is a potent fluorescent dye. Cyanine5 azide chloride can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). (λex=646 nm, λem=662 nm)[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
[1] Huppelschoten Y, et, al. Total Chemical Synthesis of a Functionalized GFP Nanobody. Chembiochem. 2022 Aug 3:e202200304. DOI:
10.1002/cbic.202200304
