Suc-Leu-Leu-Val-Tyr-AMC

Suc-Leu-Leu-Val-Tyr-AMC (94367-21-2) is a fluorogenic substrate for the chymotryptic activity of the 20S proteasome1 and other chymotrypsin-like proteases, as well as calpains2. A commonly used substrate for assaying proteasomal enzymatic activity.3,4 Excitation max.: 360 nm; emission max.: 460 nm.

Suc-Leu-Leu-Val-Tyr-AMC is a fluorgenic substrate for chymotrypsin-like proteases, as well as calpains. It can be used to treat tuberculosis.

ChEBI: A tetrapeptide compound with a succinyl group at the N-terminal and a 7-amino-4-methylcoumarin group at the C-terminal.

In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.6

[1] ROSS L. STEIN  Lawrence D  Francesco Melandri. Kinetic Characterization of the Chymotryptic Activity of the 20S Proteasome[J]. Biochemistry Biochemistry, 1996, 35 13: 3899-3908. DOI:10.1021/bi952262x
[2] T SASAKI. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates.[J]. The Journal of Biological Chemistry, 1984, 259 20: 12489-12494.
[3] MOHAMED-AMINE HAMOUDA. The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells.[J]. Oncotarget, 2014, 5 15: 6252-6266. DOI:10.18632/oncotarget.2193
[4] JIA-WEI MIN. USP14 inhibitor attenuates cerebral ischemia/reperfusion-induced neuronal injury in mice[J]. Journal of Neurochemistry, 2016, 140 5: 826-833. DOI:10.1111/jnc.13941