UBQ-2 NHS Ester

Product NameUBQ-2 NHS Ester
CAS916753-62-3
MFC29H29N7O8
MW603.58
EINECS
MOL File916753-62-3.mol

Chemical Properties

Boiling point	780.6±70.0 °C(Predicted)
Density	1.37±0.1 g/cm3(Predicted)
solubility	Easily soluble in DMSO, DMF, methanol, etc.
pka	2.91±0.50(Predicted)
Appearance	Dark purple solid
ex/em	566 nm (absorbance peak)
ε(extinction coefficient)	38000 L⋅mol−1⋅cm−1
InChIKey	UTKREZFIUDGDHQ-RRPBDJRISA-N
SMILES	N(/C1C=C(C(=CC=1OC)/N=N/C1C=CC(=CC=1)N(=O)=O)OC)=N\C1C=CC(=CC=1)N(C)CCCC(=O)ON1C(CCC1=O)=O

Usage And Synthesis

Description

UBQ-2 NHS Ester is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBQ-2 NHS Ester has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Chemical Properties

Appearance: Dark purple solid
ex/em: 566 nm (absorbance peak)
Extinction coefficient (ε): 38000 Lmol1cm1

Uses

BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Chemical Reactivity

Primary amine

Abs/Em Maxima

580 nm

Extinction Coefficient

38,000 cm-1M-1

Spectrally Similar Dyes

Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650