AF 430 is a hydrophilic dye. It is used in flow cytometry and other applications.
The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.
Appearance: Yellow solid
ex/em: 430/545 nm
Extinction coefficient (ε): 15,000 Lmol1cm1
Quantum yield (Φ): 0.23
AF430 NHS ester is an AF 430 maleimide is a derivative of the yellow fluorescent dye AF 430. AF430 has an excitation wavelength of 425 nm and an emission wavelength of 542 nm. AF430 NHS ester can be uesd for the labeling of amino-groups in peptides, proteins, and oligonucleotides. To achieve specific coupling of dye labels and biomolecules[1].
ChEBI: Alexa Fluor 430 is a methanesulfonate salt, a pyrrolidinone and an organic heterotricyclic compound. It has a role as a fluorochrome. It contains a triethylammonium ion and an Alexa Fluor 430(1-).
[1] Fernández B, Gálvez N, Sánchez P, et al. Fluorescence resonance energy transfer in ferritin labeled with multiple fluorescent dyes[J]. JBIC Journal of Biological Inorganic Chemistry, 2008, 13: 349-355.
