Mouse anti human IgE mono antibody
Mouse
WB, ELISA, Sandwich ELISA
Human
Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)
ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
ANTI-IGE抗体是一类可以特异性结合ANTI-IGE的多克隆抗体，主要用于检测ANTI-IGE的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
双抗体夹心法测定标本中ANTI-IGE水平。用纯化的ANTI-IGE抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANTI-IGE，再与HRP标记的ANTI-IGE抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANTI-IGE呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中ANTI-IGE浓度。
