Endovion (NS3728, 10-100 μM) reduces TNFα-induced apoptosis and increases p53-protein level as well as downstream signaling, eg, expression of p21 Waf1/Cip1 , Bax, Noxa, MDM2, and activation of Caspase-9/-3 in Cisplatin-sensitive cells.
Endovion (NS3728, 10 μM) inhibits cell proliferation in Capan-1, AsPC-1 and BxPC-3 cell lines.
Western Blot Analysis
| Cell Line: | Wild -type, resistant, and transiently transfected A2780 cells. |
| Concentration: | 10-100 μM. < /td> |
| Incubation Time: | 18 or 4.5 h. |
< td class="col1"> Result: Reduced the maximal taurine rate constant more than 90% compared with the untreated control cells.
Resulted i n an increased LRRC8A protein expression.
Significantly reduced p53 and p21Waf1/Cip1 protein level in A2780WT cells.
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Cell Proliferation Assay
| Cell Line: | Capan-1, AsPC-1, BxPC-3 and H6c7 cell lines. |
| Concentration: | 10 μM. |
| Incubation Time: | 24 h. |
| Result: | Resulted in the most pronounced inhibition in all cell lines with 77 ± 26 % in Capan-1, 67 ± 9 % in AsPC-1, and 54 ± 8 % in BxPC-3 cells at +67 mV. |
