IB-MECA
IB-MECA 性质
| 密度 | 1.98±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | 2-8°C |
| 溶解度 | 热乙醇：>10 mg/mL |
| 酸度系数(pKa) | 12.72±0.70(Predicted) |
| 形态 | 固体 |
| 颜色 | 白色 |
| InChIKey | HUJXGQILHAUCCV-MOROJQBDSA-N |
IB-MECA 用途与合成方法
A3AR
Piclidenoson is able to inhibit Forskolin (HY-15371)-stimulated cAMP levels with EC
50
s of 0.82 μM and 1.2 μM in OVCAR-3 cells and Caov-4 cells, respectively.
Piclidenoson (0.0001-100 μM; 48 hours) significantly reduces cell viability in a dose-dependent manner in human ovarian cancer cell lines, with IC
50
s of 32.14 μM and 45.37 μM for OVCAR-3 and Caov-4 cells, respectively.
Piclidenoson (0.001-100 μM; 48 hours) induces apoptosis in ovarian cancer cell line through the caspase pathway.
Piclidenoson induces apoptosis via the mitochondrial signaling pathway.
Cell Proliferation Assay
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.0001-100 μM |
| Incubation Time: | 48 hours |
| Result: | Resulted in a dose-dependent reduction in the cell viability. |
Apoptosis Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.1 μM, 1 μM, 10 μM, 50 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Significant increased in the percentage of apoptosis in a concentration-dependent manner. |
Western Blot Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 1 μM, 10 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased the expression of Bcl-2 was noticeably and increased the expression of Bax protein. |
Piclidenoson (105 μg/kg; i.p.) enhances survival of γ-irradiated mice.
| Animal Model: | B10CBAF1 male mice aged 3 months (average 30 g) |
| Dosage: | 105 μg/kg |
| Administration: | Intraperitoneal injection, 0.5 h after irradiation |
| Result: | Resulted in statistically significant increases of the mean survival time in comparison with the control irradiated mice. |
