1 ml 72% perchloric acid was added to a suspension of 4 g triamcinolone in
100 ml of fresh distilled acetophenone and the mixture was stirred at a room
temperature before all triamcinolone solved (for about 2 hours). The solution
was neutralized with help of 8 ml of 1.1 N sodium hydroxide and sufficient
quantity of sodium bicarbonate. Then chloroform and water were added.
Organic layer was separated and concentrated in vacuum. The residue was
recrystallized from acetone-hexane and thoroughly washed from
acetophenone with hexane. Triamcinolone acetophenide (amicinafide) was
prepared. It had MP: 281°-283°C (decomp.);αD
23 = +23°. (c = 0.98 in
CHCl3).
