(3β,5α)-3-(Acetyloxy)-pregn-16-en-20-one is a Tigogenin derivative used in the synthesis of polyhydroxy steroids; Also, it is an intermediate used in the synthesis of Allopregnenolone (A547105), which acts as a GABAA receptor positive allosteric modulator. It is a metabolite of Progesterone (P755900). s It is also a neuroactive steroid present in the blood and also the brain.