Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.
Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.
The NT-3 is purified by proprietary chromatographic techniques.
NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4. NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes. NT-3 is required for the development of peripheral sensory neurons. NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form. NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB.
[1] Huang, E.J. and Reichardt, L.F. (2001) Annu Rev Neurosci 24, 677-736.
[2] Noga, O. et al. (2003) Clin Exp Allergy 33, 649-54.
[3] Truzzi, F. et al. (2008) J Invest Dermatol 128, 2031-40.
[4] Tessarollo, L. et al. (1994) Proc Natl Acad Sci U S A 91, 11844-8.
