H-GLY-PRO-ARG-PRO-NH2

Gly-Pro-Arg-Pro amide has been used-

• as a constituent of buffer during fluid-phase and bound thrombin inhibition assays, to determine the activity of thrombin
• for blocking fibrin polymerization and preventing fibrin clot formation in presence of α-thrombin, in blood obtained from mice
• during preparation of human soluble fibrin, to block the polymerization of soluble fibrin

Gly-Pro-Arg-Pro is a peptide that mimics the N-terminal Gly-Pro-Arg region in the a chain of fibrin protein.

Gly-Pro-Arg-Pro peptide suppresses the early steps of fibrin polymerization. The amide derivative of this peptide prevents the degradation of fibrinogen and fragment D by plasmin, even in the presence of EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid).