4-hydroxy Nonenal Mercapturic Acid-d3

4-hydroxy Nonenal mercapturic acid-d3 contains three deuterium atoms at the terminal methyl position. It is intended for use as an internal standard for the quantification of 4-HNE mercapturic acid by GC- or LC-mass spectrometry. Peroxidation of common ω-6 polyunsaturated fatty acids (PUFAs) such as linoleic acid, DGLA, and arachidonic acid can give rise to 4-HNE. 4-HNE is cleared rapidly from the plasma and undergoes enterohepatic circulation as a glutathione conjugate in the rat.1 About two thirds of an administered dose of 4-HNE is excreted within 48 hours in the urine, primarily in the form of mercapturic acid conjugates.2 The C-1 aldehyde of 4-HNE is reduced to an alcohol in about half of these metabolites. The remainder are C-1 aldehydes or have been oxidized to C-1 carboxylic acids. These aldehydes and carboxylic acids can also form γ-lactols and γ-lactones, respectively, producing at least four or five end urinary metabolites of 4-HNE in vivo.WARNING This product is not for human or veterinary use.

[1] ALEXIA LAURENT. Analysis in the Rat of 4-Hydroxynonenal Metabolites Excreted in Bile: Evidence of Enterohepatic Circulation of These Byproducts of Lipid Peroxidation[J]. Chemical Research in Toxicology, 1999, 12 10: 887-894. DOI: 10.1021/tx9900425
[2] JACQUES ALARY. Mercapturic Acid Conjugates as Urinary End Metabolites of the Lipid Peroxidation Product 4-Hydroxy-2-nonenal in the Rat[J]. Chemical Research in Toxicology, 1995, 8 1: 34-39. DOI: 10.1021/tx00043a004