单氯二胺
![单氯二胺 结构式](https://img.chemicalbook.com/CAS/GIF/76421-73-3.gif)
单氯二胺 性质
熔点 | 135-136 °C(lit.) |
---|---|
沸点 | 314.9±44.0 °C(Predicted) |
密度 | 1.40±0.1 g/cm3(Predicted) |
储存条件 | Keep in dark place,Inert atmosphere,2-8°C |
溶解度 | 在DMF中可溶 |
酸度系数(pKa) | -3.22±0.70(Predicted) |
形态 | 固体 |
颜色 | 浅黄至黄色 |
BRN | 4440901 |
单氯二胺 用途与合成方法
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
The experiment should consist of parallel negative, positive and experimental wells respectively.
Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 µM H
2
O
2
)
2. Incubate the plate overnight at 37 °C, 5 % CO
2
. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of
20-100 μM
from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 µL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 µL of the culture suspension into the white opaque plate and record fluorescence at E
X
/E
M
= 380/465 ±20 nm respectively (
blue
).
单氯二胺 价格(试剂级)
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024-04-30 | HY-101899 | 1 mg | 305 | ||
2024-04-30 | HY-101899 | CHLOROBIMANE | 76421-73-3 | 5mg | 610 |